Results
| PMID | 25673457 |
| Gene Name | ATP1B4 |
| Condition | Endometriosis |
| Association |
Associated |
| Population size | 124 |
| Population details | 124 (50 patients with endometriosis, 34 with benign ovarian neoplasms, 40 healthy volunteers) |
| Age | Patients: 18–51 yrs, Controls: 23–54 yrs |
| Sex | Female |
| Other associated phenotypes |
Endometriosis (EM) |
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Chin Med J (Engl). 2015 Feb 20;128(4):520-7. doi: 10.4103/0366-6999.151108. Zhao, Yang| Liu, Ya-Nan| Li, Yi| Tian, Li| Ye, Xue| Cui, Heng| Chang, Xiao-Hong Gynecology Oncology Center, Peking University People's Hospital, Beijing 100044, China. BACKGROUND: We investigated possible biomarkers for endometriosis (EM) using the ClinProt technique and proteomics methods. METHODS: We enrolled 50 patients with EM, 34 with benign ovarian neoplasms and 40 healthy volunteers in this study. Serum proteomic spectra were generated by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MS) combined with weak cationic exchange (WCX) magnetic beads. Possible biomarkers were analyzed by a random and repeat pattern model-validation method that we designed, and ClinProtools software, results were refined using online liquid chromatography-tandem MS. RESULTS: We found a cluster of 5 peptides (4210, 5264, 2660, 5635, and 5904 Da), using 3 peptides (4210, 5904, 2660 Da) to discriminate EM patients from healthy volunteers, with 96.67% sensitivity and 100% specificity. We selected 4210 and 5904 m/z, which differed most between patients with EM and controls, and identified them as fragments of ATP1B4, and the fibrinogen alpha (FGA) isoform 1/2 of the FGA chain precursor, respectively. CONCLUSIONS: ClinProt can identify EM biomarkers, which - most notably - distinguish even early-stage or minimal disease. We found 5 stable peaks at 4210, 5264, 2660, 5635, and 5904 Da as potential EM biomarkers, the strongest of which were associated with ATP1B4 (4210 Da) and FGA (5904 Da); this indicates that ATP1B4 and FGA are associated with EM pathogenesis. Mesh Terms: Adolescent| Adult| Biomarkers/*blood| Endometriosis/*blood/*diagnosis| Female| Humans| Middle Aged| Peptides/blood| Proteomics/*methods| Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization| Young Adult|DA 2015/09/29 06:00 |
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